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| Phonsakhan,Wipada; Kong-Ngern,Kanlaya. |
| Background Black glutinous rice contains remarkable levels of anthocyanins, which possess anti-oxidative properties and thus have health benefits. The accumulation of anthocyanins in grains of thirty black glutinous rice varieties was measured, and the results revealed that the accumulated anthocyanin content ranged from 0.262 to 2.539 mg/g. Black glutinous rice Br no. 19 was selected, and its leaf protein expression profile was compared with that of white glutinous rice RD 6 using 2D-PAGE, and the protein spots were then directly analyzed after proteolysis by LC-MS/MS. Results The proteins from the leaves of the two rice varieties were separated using 2D-PAGE and silver stained, and the spots were analyzed using Image Master 2D Platinum version 5... |
| Tipo: Journal article |
Palavras-chave: 2D-PAGE; Anthocyanin; LC-MS/MS; Oryza sativa L; Proteomics. |
| Ano: 2015 |
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582015000100006 |
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| SILVA,NATHALIA A.; ALBUQUERQUE,CLEIDE M.R.; MARINHO,ALINE D.; JORGE,ROBERTA J.B.; SILVA NETO,ANTONIO G.; MONTEIRO,HELENA S.A.; SILVA,TÚLIO D.; SILVA,MÁRCIA V.; CORREIA,MARIA TEREZA S.; PEREIRA,TICIANA P.; MARTINS,ALICE M.C.; MENEZES,DALGIMAR B.; XIMENES,RAFAEL M.; MARTINS,RENÉ D.. |
| ABSTRACT Scorpions belonging to the Tityus genus are of medical interest in Brazil. Among them, Tityus stigmurus is the main scorpion responsible for stings in the Northeast region. After a sting, the scorpion venom distributes rapidly to the organs, reaching the kidneys quickly. However, there are few studies concerning the renal pathophysiology of scorpion poisoning. In this study, we evaluated the effects of T. stigmurus venom (TsV) on renal parameters in isolated rat kidneys. Wistar rats (n = 6), weighing 250-300 g, were perfused with Krebs-Henseleit solution containing 6 g/100 mL bovine serum albumin. TsV at 0.3 and 1.0 μg/mL was tested, and the effects on perfusion pressure (PP), renal vascular resistance (RVR), urinary flow (UF), glomerular... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: 2D-PAGE; Kidney; MDCK; Venom; Tityus stigmurus. |
| Ano: 2016 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652016000200665 |
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